MediumNCTC-109 is a chemically defined culture medium developed at the NCTC in London in the early 1950s and represents one of the first defined formulations capable of supporting mammalian cell survival without natural extracts. It contains a basic combination of amino acids, vitamins, salts, and carbohydrates, making it suitable primarily for short-term survival studies, metabolic research, and cytogenetic applications. Although largely of historical significance today,NCTC-109 played a key role in advancing the understanding of essential nutrient requirements for mammalian cells in vitro.

Applications

• Routine maintenance and propagation of mammalian cell lines

• Cytogenetic studies requiring reproducible conditions

• Viral culture and propagation in chemically defined medium

• In vitro assays requiring low variability and consistency

• Studies in metabolism, growth kinetics, and media optimization

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