With High Glucose and Sodium Pyruvate. Without L-Glutamine
Cells effectively cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. DMEM is also commonly used with cell lines such as HeLa, 293, Cos-7, and PC-12, amongst others. DMEM is a widely used basal medium for supporting the growth of many different mammalian cells.