SERANA FBS:

Examples of Application for Different Cell Lines

The application of Serana's Fetal Bovine Serum (FBS) products in cell culture of more than a hundred different cell lines has been reported in several hundreds of publications in prestigious peer-reviewed journals. The used cell lines include human origin, animal origin, and different micro-organisms. Below we have listed some recent publications and the used cell lines. Please do not hesitate to contact us if you may have questions regarding the usage of our products for any specific cell line.

Dr. Iman Kamranfar
Quality Assurance Manager
Serana Europe GmbH
Date: 26/04/2022

RPE-1 cells: hTert-immortalized human retinal pigment epithelium nontumoral HMEC cells: hTert-immortalized human mammary epithelial nontumoral cells

Diosmetin Protects against Cardiac Hypertrophy via p62/Keap1/Nrf2 Signaling Pathway. Oxid Med Cell Longev. 2;2022:8367997.

Development of a sandwich enzyme-linked immunosorbent assay (ELISA) to quantify γ-glutamyl-carboxylated clotting factor IX and assess redox susceptibility of anticoagulant chemicals. Journal of Veterinary Medical Science, 22-0079.

Fecal Filobasidium Is Associated with Clinical Remission and Endoscopic Response following Fecal Microbiota Transplantation in Mild-to-Moderate Ulcerative Colitis. Microorganisms, 10(4), 737.

Calu-3: human lung cancer cell, Caco-2: human colorectal epithelial adenocarcinoma cells, and Vero cells: green African monkey kidney epithelial cells

ADAP Y571 Phosphorylation Is Required to Prime STAT3 for Activation in TLR4-Stimulated Macrophages. The Journal of Immunology, 206(4), 814-826.

Long non coding RNA SNHG3 promotes the development of non small cell lung cancer via the miR 1343 3p/NFIX pathway. International Journal of Molecular Medicine, 48, 147.

A BRCA1 coiled-coil domain variant disrupting PALB2 interaction promotes the development of mammary tumors and confers a targetable defect in homologous recombination repair. Cancer Research, 81(24), 6171-6182.

Dual Mechanisms of Cardiac Action Potential Prolongation by 4-Oxo-Nonenal Increasing the Risk of Arrhythmia; Late Na+ Current Induction and hERG K+ Channel Inhibition. Antioxidants. 10(7):1139.

Higher expression of KCNK10 (TREK-2) K+ channels and their functional upregulation by lipopolysaccharide treatment in mouse peritoneal B1a cells. Pflugers Arch - Eur J Physiol 473, 659–671

Immunization of chicken with flagellin adjuvanted Salmonella enteritidis bacterial ghosts confers complete protection against chicken salmonellosis. Poultry science, 100(7), 101205.

Single oral immunization of an attenuated Salmonella Gallinarium formulation consisting of equal quantities of strains secreting H9N2 hemagglutinin-HA1, HA2, and M2eCD154. Veterinary Immunology and Immunopathology, 240, 110318.

A kinome-centered CRISPR-Cas9 screen identifies activated BRAF to modulate enzalutamide resistance with potential therapeutic implications in BRAF-mutated prostate cancer. Scientific reports, 11(1), 1-8.

Lung adenocarcinoma cells: SKBR3, HCC827, H3255, H3122, H2228, human breast cancer cell lines: SKBR3 and HCC1954, BJ human fibroblast cells, RPE1 hTERT-immortalized retinal pigment epithelial cells, human colorectal carcinoma cell lines: DiFi and Lim1215.

Multiple low dose therapy as an effective strategy to treat EGFR inhibitor-resistant NSCLC tumours. Nature communications, 11(1), 1-9.

lncRNA KCNQ1OT1 knockdown inhibits colorectal cancer cell proliferation, migration and invasiveness via thePI3K/AKT pathway. Oncology Letters, 20, 601-610.

Genipin enhances the antitumor effect of elesclomol in A549 lung cancer cells by blocking uncoupling protein‑2 and stimulating reactive oxygen species production. Oncology Letters, 20, 374.

Effects of a Biotinylated Squamocin Molecule on Apoptosis in Human Nasopharyngeal Carcinoma Cells. International Journal of Science. 7, 62-69.

Effects of miR 218 1 3p and miR 149 on proliferation and apoptosis of non small cell lung cancer cells. Oncology Letters, 20, 96.

ITCH regulates oxidative stress induced by high glucose through thioredoxin interacting protein in cultured human lens epithelial cells. Molecular Medicine Reports, 22, 4307-4319. https://doi.org/10.3892/mmr.2020.11499

ω-hydroxyundec-9-enoic acid induction of breast cancer cells apoptosis through generation of mitochondrial ROS and phosphorylation of AMPK. Archives of Pharmacal Research, 43(7), 735-743.

Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2. Cancer letters, 469, 22-34.

Targeting poor proteasomal function with radioiodine eliminates CT26 colon cancer stem cells resistant to bortezomib therapy. Scientific reports, 10(1), 1-13.

Recombinant CsHscB of carcinogenic liver fluke Clonorchis sinensis induces IL-10 production by binding with TLR2. PLoS Neglected Tropical Diseases, 14(10), e0008643.

Anticancer activity of Momordica cochinchinensis (red gac) aril and the impact of varietal diversity. BMC Complementary Medicine and Therapies, 20(1), 1-15.

Development of novel chemically-modified nucleic acid molecules for efficient inhibition of human MAPT gene expression. Genes, 11(6), 667.

Targeting poor proteasomal function with radioiodine eliminates CT26 colon cancer stem cells resistant to bortezomib therapy. Scientific reports, 10(1), 1-13.

MHCC97H: human hepatocellular carcinoma, A549: human lung cancer cell line, HCC1937: human triple negative breast cancer cell line

Preparation and antitumor activity of selenium-modified glucomannan oligosaccharides. Journal of Functional Foods, 65, 103731.

In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like Characteristics. Sci Rep 9, 18561 (2019).

The human glioma cell lines U87MG (p53wt, PTENmut, p14ARF/p16del) and LN229 (p53mut, PTENwt, p14ARF/p16del) BV2 cells (murine microglia)

Ly6G+ inflammatory cells enable the conversion of cancer cells to cancer stem cells in an irradiated glioblastoma model. Cell Death & Differentiation, 26(10), 2139-2156.

A chemotaxis model to explain WHIM neutrophil accumulation in the bone marrow of WHIM mouse model. Blood Science, 1(01), 102-112.

Fabrication and characterization of electrospun silk fibroin/gelatin scaffolds crosslinked with glutaraldehyde vapor. Frontiers in Materials, 6, 91.

Aflatoxin B1 induces immunotoxicity through the DNA methyltransferase-mediated JAK2/STAT3 pathway in 3D4/21 cells. Journal of agricultural and food chemistry, 67(13), 3772-3780.

Developmental regulation of type 1 and type 3 interferon production and risk for infant infections and asthma development. Journal of Allergy and Clinical Immunology, 143(3), 1176-1182.

ATP-binding cassette transporters substantially reduce estimates of ALDH-positive Cancer cells based on Aldefluor and AldeRed588 assays. Scientific reports, 9(1), 1-9.

Human Colorectal Cancer cell lines (HCT116), Primary Normal Human Dermal Fibroblast Adult Cells (HDFa cells), Human bone-marrow derived Mesenchymal Stem Cells (hMSC)

ATP-binding cassette transporters substantially reduce estimates of ALDH-positive Cancer cells based on Aldefluor and AldeRed588 assays. Scientific reports, 9(1), 1-9.

Characterization of the ERG-regulated kinome in prostate cancer identifies TNIK as a potential therapeutic target. Neoplasia, 21(4), 389-400.

HepG2 human hepatocellular carcinoma cells, MDA-MB-231 human breast carcinoma cells, BRIN-BD11 rat insulinoma cells, THP-1 human leukemic monocytes

The novel high-frequency variant of TRPV3 p. A628T in East Asians showing faster sensitization in response to chemical agonists. Pflügers Archiv-European Journal of Physiology, 471(10), 1273-1289.

FGF23-Mediated Activation of Local RAAS Promotes Cardiac Hypertrophy and Fibrosis. International Journal of Molecular Sciences. 2019; 20(18):4634

A rise in ATP, ROS, and mitochondrial content upon glucose withdrawal correlates with a dysregulated mitochondria turnover mediated by the activation of the protein deacetylase SIRT1. Cells, 8(1), 11.

Bisphenol AF-induced endogenous transcription is mediated by ERα and ERK1/2 activation in human breast cancer cells. PloS one, 9(4), e94725.

SERANA FBS:

Examples of Application for Different Cell Lines

RPE-1 cells: hTert-immortalized human retinal pigment epithelium nontumoral HMEC cells: hTert-immortalized human mammary epithelial nontumoral cells

Unexpected gene activation following CRISPR‐Cas9‐mediated genome editing. EMBO reports, 23(2), e53902.

Manjón, A. G., Linder, S., Teunissen, H., Friskes, A., Zwart, W., de Wit, E., & Medema, R. H.

2022

https://www.embopress.org/doi/full/10.15252/embr.202153902

Neonatal Rat Ventricular Myocytes

Diosmetin Protects against Cardiac Hypertrophy via p62/Keap1/Nrf2 Signaling Pathway. Oxid Med Cell Longev. 2;2022:8367997.

Guo Y, Li D, Cen XF, Qiu HL, Ma YL, Liu Y, Huang SH, Liu LB, Xu M, Tang QZ.

2022

https://pubmed.ncbi.nlm.nih.gov/35242278/

HEK293T cells

Development of a sandwich enzyme-linked immunosorbent assay (ELISA) to quantify γ-glutamyl-carboxylated clotting factor IX and assess redox susceptibility of anticoagulant chemicals. Journal of Veterinary Medical Science, 22-0079.

SATO, R., WATANABE, K., KAMATA, R., & TAKEDA, K.

2022

https://www.jstage.jst.go.jp/article/jvms/advpub/0/advpub_22-0079/_article/-char/ja/

Human monocytes, HT29 epithelial cells

Fecal Filobasidium Is Associated with Clinical Remission and Endoscopic Response following Fecal Microbiota Transplantation in Mild-to-Moderate Ulcerative Colitis. Microorganisms, 10(4), 737.

van Thiel, I. A., Rahman, S., Hakvoort, T. B., Davids, M., Verseijden, C., van Hamersveld, P. H., ... & de Jonge, W. J.

2022

https://www.mdpi.com/2076-2607/10/4/737

Human monocytes, HT29 epithelial cells

Fecal Filobasidium Is Associated with Clinical Remission and Endoscopic Response following Fecal Microbiota Transplantation in Mild-to-Moderate Ulcerative Colitis. Microorganisms, 10(4), 737.

van Thiel, I. A., Rahman, S., Hakvoort, T. B., Davids, M., Verseijden, C., van Hamersveld, P. H., ... & de Jonge, W. J.

2022

https://www.mdpi.com/2076-2607/10/4/737

Calu-3: human lung cancer cell, Caco-2: human colorectal epithelial adenocarcinoma cells, and Vero cells: green African monkey kidney epithelial cells

A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome. Nature communications, 12(1), 1-16.

Kim, D., Kim, S., Park, J., Chang, H. R., Chang, J., Ahn, J., ... & Baek, D.

2021

https://www.nature.com/articles/s41467-021-25361-5

hTert-immortalized retinal pigment epithelium (RPE)

Resistance of Hypoxic Cells to Ionizing Radiation Is Mediated in Part via Hypoxia-Induced Quiescence. Cells. 10(3):610.

Menegakis A, Klompmaker R, Vennin C, Arbusà A, Damen M, van den Broek B, Zips D, van Rheenen J, Krenning L, Medema RH.

2021

https://www.mdpi.com/2073-4409/10/3/610

Bone marrow–derived macrophages (BMMs)

ADAP Y571 Phosphorylation Is Required to Prime STAT3 for Activation in TLR4-Stimulated Macrophages. The Journal of Immunology, 206(4), 814-826.

Yang, N., Xiong, Y., Wang, Y., Yi, Y., Zhu, J., Ma, F., ... & Liu, H.

2021

https://pubmed.ncbi.nlm.nih.gov/33431658/

The human normal lung cell line BEAS-2B and the human NSCLC cell lines (H1299, H358, A549 and H1975)

Long non coding RNA SNHG3 promotes the development of non small cell lung cancer via the miR 1343 3p/NFIX pathway. International Journal of Molecular Medicine, 48, 147.

Zhao, L., Song, X., Guo, Y., Ding, N., Wang, T., & Huang, L.

2021

https://www.spandidos-publications.com/10.3892/ijmm.2021.4980

Human breast cancer cell line MCF-7

A BRCA1 coiled-coil domain variant disrupting PALB2 interaction promotes the development of mammary tumors and confers a targetable defect in homologous recombination repair. Cancer Research, 81(24), 6171-6182.

Pulver, E. M., Mukherjee, C., van de Kamp, G., Roobol, S. J., Rother, M. B., van der Gulden, H., ... & Jonkers, J.

2021

https://pubmed.ncbi.nlm.nih.gov/34548335/

HEK-293 and e hERG-HEK cell lines

Dual Mechanisms of Cardiac Action Potential Prolongation by 4-Oxo-Nonenal Increasing the Risk of Arrhythmia; Late Na+ Current Induction and hERG K+ Channel Inhibition. Antioxidants. 10(7):1139.

Choi S-W, Yin M-Z, Park N-K, Woo J-H, Kim S-J.

2021

https://www.mdpi.com/2076-3921/10/7/1139

Mouse primary B cells

Higher expression of KCNK10 (TREK-2) K+ channels and their functional upregulation by lipopolysaccharide treatment in mouse peritoneal B1a cells. Pflugers Arch - Eur J Physiol 473, 659–671

Choi, S.W., Woo, J., Park, K.S. et al.

2021

https://link.springer.com/article/10.1007/s00424-021-02526-1

Vero, Calu-3, and Madin-Darby canine kidney (MDCK) cells

Antiviral efficacy of pralatrexate against sars-cov-2. Biomolecules & Therapeutics, 29(3), 268.

Bae, J. Y., Lee, G. E., Park, H., Cho, J., Kim, J., Lee, J., ... & Park, M. S.

2021

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094065/

Peripheral Blood Mononuclear Cells (PBMC), Human Embryonic Kidney (HEK293T) cells

Immunization of chicken with flagellin adjuvanted Salmonella enteritidis bacterial ghosts confers complete protection against chicken salmonellosis. Poultry science, 100(7), 101205.

Senevirathne, A., Hewawaduge, C., & Lee, J. H.

2021

https://www.sciencedirect.com/science/article/pii/S1756464619306553

Chicken Peripheral blood mononuclear cell

Single oral immunization of an attenuated Salmonella Gallinarium formulation consisting of equal quantities of strains secreting H9N2 hemagglutinin-HA1, HA2, and M2eCD154. Veterinary Immunology and Immunopathology, 240, 110318.

Senevirathne, A., Hewawaduge, C., Park, S., Jawalagatti, V., Kim, C., Seo, B. J., ... & Lee, J. H.

2021

https://www.sciencedirect.com/science/article/abs/pii/S0165242721001367

Vero E6 cells (green african monkey epithelial kidney cells)

Copper-impregnated three-layer mask efficiently inactivates SARS-CoV2. Environmental Research, 196, 110947.

Hewawaduge, C., Senevirathne, A., Jawalagatti, V., Kim, J. W., & Lee, J. H.